c tropicalis atcc 20336 genomic dna (ATCC)
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c tropicalis atcc 20336 genomic dna
C Tropicalis Atcc 20336 Genomic Dna, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+tropicalis+atcc+20336+genomic+dna/Candida+viswanathii+Sandhu+et+Randhawa/pm31654413-110-32-34
Average 96 stars, based on 124 article reviews
C Tropicalis Atcc 20336 Genomic Dna, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+tropicalis+atcc+20336+genomic+dna/Candida+viswanathii+Sandhu+et+Randhawa/pm31654413-110-32-34
Average 96 stars, based on 124 article reviews
c tropicalis atcc 20336 genomic dna - by Bioz Stars,
2026-09
96/100 stars
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Polymerase Chain Reaction:Article Title: Identification and Characterization of the CYP52 Family of Candida tropicalis ATCC 20336, Important for the Conversion of Fatty Acids and Alkanes to ?,?-Dicarboxylic Acids Article Snippet: .. With Article Title: Enhancement of xylitol production by attenuation of intracellular xylitol dehydrogenase activity in Candida tropicalis. Article Snippet: To construct Candida tropicalis strains that produce a high yield of xylitol with no requirement for co-substrates, we engineered the yeast with an attenuated xylitol dehydrogenase (XDH) and then assessed the efficiency of xylitol production The mutants, strains XDH-5 (with only one copy of the XDH gene), and ARSdR-16 (with a mutated XDH gene) showed 70 and 40% of wild type (WT) XDH activity, respectively.. Conversions of xylose to xylitol by WT, XDH-5, and ARSdR-16 were 62, 64, and 75%, respectively, with productivities of 0.52, 0.54, and 0.62 g l h, respectively.. The ARSdR-16 mutant strain produced xylitol with high yield and high productivity in a simple process that required no co-substrates, such as glycerol. Article Title: Fatty alcohol oxidase genes and proteins from Article Snippet: .. Article Title: Identification and Characterization of the CYP52 Family of Candida tropicalis ATCC 20336, Important for the Conversion of Fatty Acids and Alkanes to α,ω-Dicarboxylic Acids Article Snippet: .. Amplification of Article Title: Development of an efficient genetic manipulation strategy for sequential gene disruption and expression of different heterologous GFP genes in Candida tropicalis. Article Snippet: The diploid yeast Candida tropicalis, which can utilize n-alkane as a carbon and energy source, is an attractive strain for both physiological studies and practical applications.. However, it presents some characteristics, such as rare codon usage, difficulty in sequential gene disruption, and inefficiency in foreign gene expression, that hamper strain improvement through genetic engineering.. In this work, we present a simple and effective method for sequential gene disruption in C. tropicalis based on the use of an auxotrophic mutant host defective in orotidine monophosphate decarboxylase (URA3). Article Title: A CRISPR-Cas9 system for multiple genome editing and pathway assembly in Candida tropicalis. Article Snippet: A cc ep te d A rt ic le Genetic manipulation is among the most important tools for synthetic biology; however, modifying multiple genes is extremely time-consuming and can sometimes be impossible when dealing with gene families.. Here, we present a CRISPR-Cas9 system for use in the diploid yeast Candida tropicalis that is vastly superior to traditional techniques.. This system enables the rapid and reliable introduction of multiple genetic deletions or mutations, as well as stable expression using an integrated CRISPR-Cas9 cassette or a transient CRISPR–Cas9 cassette, together with a short donor DNA. Amplification:Article Title: Identification and Characterization of the CYP52 Family of Candida tropicalis ATCC 20336, Important for the Conversion of Fatty Acids and Alkanes to ?,?-Dicarboxylic Acids Article Snippet: .. With Article Title: Enhancement of xylitol production by attenuation of intracellular xylitol dehydrogenase activity in Candida tropicalis. Article Snippet: To construct Candida tropicalis strains that produce a high yield of xylitol with no requirement for co-substrates, we engineered the yeast with an attenuated xylitol dehydrogenase (XDH) and then assessed the efficiency of xylitol production The mutants, strains XDH-5 (with only one copy of the XDH gene), and ARSdR-16 (with a mutated XDH gene) showed 70 and 40% of wild type (WT) XDH activity, respectively.. Conversions of xylose to xylitol by WT, XDH-5, and ARSdR-16 were 62, 64, and 75%, respectively, with productivities of 0.52, 0.54, and 0.62 g l h, respectively.. The ARSdR-16 mutant strain produced xylitol with high yield and high productivity in a simple process that required no co-substrates, such as glycerol. Article Title: Identification and Characterization of the CYP52 Family of Candida tropicalis ATCC 20336, Important for the Conversion of Fatty Acids and Alkanes to α,ω-Dicarboxylic Acids Article Snippet: .. Amplification of Article Title: A CRISPR-Cas9 system for multiple genome editing and pathway assembly in Candida tropicalis. Article Snippet: A cc ep te d A rt ic le Genetic manipulation is among the most important tools for synthetic biology; however, modifying multiple genes is extremely time-consuming and can sometimes be impossible when dealing with gene families.. Here, we present a CRISPR-Cas9 system for use in the diploid yeast Candida tropicalis that is vastly superior to traditional techniques.. This system enables the rapid and reliable introduction of multiple genetic deletions or mutations, as well as stable expression using an integrated CRISPR-Cas9 cassette or a transient CRISPR–Cas9 cassette, together with a short donor DNA. Purification:Article Title: Identification and Characterization of the CYP52 Family of Candida tropicalis ATCC 20336, Important for the Conversion of Fatty Acids and Alkanes to ?,?-Dicarboxylic Acids Article Snippet: Membranes were dried overnight before hybridizing to oligonucleotide probes prepared with a nonradioactive enhanced chemiluminescence (ECL) 3′ oligolabeling and detection system (Amersham Life Sciences). .. Agarose Gel Electrophoresis:Article Title: Identification and Characterization of the CYP52 Family of Candida tropicalis ATCC 20336, Important for the Conversion of Fatty Acids and Alkanes to ?,?-Dicarboxylic Acids Article Snippet: Membranes were dried overnight before hybridizing to oligonucleotide probes prepared with a nonradioactive enhanced chemiluminescence (ECL) 3′ oligolabeling and detection system (Amersham Life Sciences). .. Electrophoresis:Article Title: Identification and Characterization of the CYP52 Family of Candida tropicalis ATCC 20336, Important for the Conversion of Fatty Acids and Alkanes to ?,?-Dicarboxylic Acids Article Snippet: Membranes were dried overnight before hybridizing to oligonucleotide probes prepared with a nonradioactive enhanced chemiluminescence (ECL) 3′ oligolabeling and detection system (Amersham Life Sciences). .. Expressing:Article Title: A CRISPR-Cas9 system for multiple genome editing and pathway assembly in Candida tropicalis. Article Snippet: A cc ep te d A rt ic le Genetic manipulation is among the most important tools for synthetic biology; however, modifying multiple genes is extremely time-consuming and can sometimes be impossible when dealing with gene families.. Here, we present a CRISPR-Cas9 system for use in the diploid yeast Candida tropicalis that is vastly superior to traditional techniques.. This system enables the rapid and reliable introduction of multiple genetic deletions or mutations, as well as stable expression using an integrated CRISPR-Cas9 cassette or a transient CRISPR–Cas9 cassette, together with a short donor DNA. Clone Assay:Article Title: A CRISPR-Cas9 system for multiple genome editing and pathway assembly in Candida tropicalis. Article Snippet: A cc ep te d A rt ic le Genetic manipulation is among the most important tools for synthetic biology; however, modifying multiple genes is extremely time-consuming and can sometimes be impossible when dealing with gene families.. Here, we present a CRISPR-Cas9 system for use in the diploid yeast Candida tropicalis that is vastly superior to traditional techniques.. This system enables the rapid and reliable introduction of multiple genetic deletions or mutations, as well as stable expression using an integrated CRISPR-Cas9 cassette or a transient CRISPR–Cas9 cassette, together with a short donor DNA. Construct:Article Title: A CRISPR-Cas9 system for multiple genome editing and pathway assembly in Candida tropicalis. Article Snippet: A cc ep te d A rt ic le Genetic manipulation is among the most important tools for synthetic biology; however, modifying multiple genes is extremely time-consuming and can sometimes be impossible when dealing with gene families.. Here, we present a CRISPR-Cas9 system for use in the diploid yeast Candida tropicalis that is vastly superior to traditional techniques.. This system enables the rapid and reliable introduction of multiple genetic deletions or mutations, as well as stable expression using an integrated CRISPR-Cas9 cassette or a transient CRISPR–Cas9 cassette, together with a short donor DNA. Control:Article Title: Fatty alcohol oxidase genes and proteins from Article Snippet: .. Article Title: Development of an efficient genetic manipulation strategy for sequential gene disruption and expression of different heterologous GFP genes in Candida tropicalis. Article Snippet: The diploid yeast Candida tropicalis, which can utilize n-alkane as a carbon and energy source, is an attractive strain for both physiological studies and practical applications.. However, it presents some characteristics, such as rare codon usage, difficulty in sequential gene disruption, and inefficiency in foreign gene expression, that hamper strain improvement through genetic engineering.. In this work, we present a simple and effective method for sequential gene disruption in C. tropicalis based on the use of an auxotrophic mutant host defective in orotidine monophosphate decarboxylase (URA3). Derivative Assay:Article Title: Development of an efficient genetic manipulation strategy for sequential gene disruption and expression of different heterologous GFP genes in Candida tropicalis. Article Snippet: The diploid yeast Candida tropicalis, which can utilize n-alkane as a carbon and energy source, is an attractive strain for both physiological studies and practical applications.. However, it presents some characteristics, such as rare codon usage, difficulty in sequential gene disruption, and inefficiency in foreign gene expression, that hamper strain improvement through genetic engineering.. In this work, we present a simple and effective method for sequential gene disruption in C. tropicalis based on the use of an auxotrophic mutant host defective in orotidine monophosphate decarboxylase (URA3). Plasmid Preparation:Article Title: A CRISPR-Cas9 system for multiple genome editing and pathway assembly in Candida tropicalis. Article Snippet: A cc ep te d A rt ic le Genetic manipulation is among the most important tools for synthetic biology; however, modifying multiple genes is extremely time-consuming and can sometimes be impossible when dealing with gene families.. Here, we present a CRISPR-Cas9 system for use in the diploid yeast Candida tropicalis that is vastly superior to traditional techniques.. This system enables the rapid and reliable introduction of multiple genetic deletions or mutations, as well as stable expression using an integrated CRISPR-Cas9 cassette or a transient CRISPR–Cas9 cassette, together with a short donor DNA. |